A RAB family antigen of Angiostrongylus cantonensis induces a Th1-type immune response in vitro and in vivo.
Exp Parasitol
; 134(1): 122-7, 2013 May.
Article
em En
| MEDLINE
| ID: mdl-23458235
ABSTRACT
A cDNA library was constructed from an Angiostrongylus cantonensis young adult and the encoded proteins were expressed in Escherichia coli. One reactive antigen, a RAB-2 protein, was selected using an immunoscreening technique. The expression of the Th1-type cytokine IFN-γ was elicited in mouse splenic cells that were co-cultured with the recombinant RAB-2 protein and in the sera of mice that were immunised with this protein and adjuvant (50 µg at 2-week intervals). In the A. cantonensis-infected groups, the mice were orally infected with 35 infective larvae, and a subset of the infected mice were immunised with the recombinant RAB-2 protein in adjuvant. Serum samples were collected every week for ELISA, and the pathological examinations were performed at 14 days post infection (dpi). An increase in IFN-γ expression was noted in the blood, and the brain sections revealed moderate eosinophilic meningitis in the immunised mice. The RAB-2 antigen of A. cantonensis induced a Th1-type immune response both in vitro and in vivo.
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Base de dados:
MEDLINE
Assunto principal:
Infecções por Strongylida
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Angiostrongylus cantonensis
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Células Th1
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Proteína rab2 de Ligação ao GTP
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Antígenos de Helmintos
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article