Caged mono- and divalent ligands for light-assisted disruption of PDZ domain-mediated interactions.
J Am Chem Soc
; 135(12): 4580-3, 2013 Mar 27.
Article
em En
| MEDLINE
| ID: mdl-23480637
ABSTRACT
We report a general method for light-assisted control of interactions of PDZ domain binding motifs with their cognate domains by the incorporation of a photolabile caging group onto the essential C-terminal carboxylate binding determinant of the motif. The strategy was implemented and validated for both simple monovalent and biomimetic divalent ligands, which have recently been established as powerful tools for acute perturbation of native PDZ domain-dependent interactions in live cells.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Domínios PDZ
/
Corantes Fluorescentes
Limite:
Animals
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article