Intra-species bacterial quorum sensing studied at single cell level in a double droplet trapping system.
Int J Mol Sci
; 14(5): 10570-81, 2013 May 21.
Article
em En
| MEDLINE
| ID: mdl-23698779
ABSTRACT
In this paper, we investigated the intra-species bacterial quorum sensing at the single cell level using a double droplet trapping system. Escherichia coli transformed to express the quorum sensing receptor protein, LasR, were encapsulated in microdroplets that were positioned adjacent to microdroplets containing the autoinducer, N-(3-oxododecanoyl)-L-homoserine lactone (OdDHL). Functional activation of the LasR protein by diffusion of the OdDHL across the droplet interface was measured by monitoring the expression of green fluorescent protein (GFP) from a LasR-dependent promoter. A threshold concentration of OdDHL was found to induce production of quorum-sensing associated GFP by E. coli. Additionally, we demonstrated that LasR-dependent activation of GFP expression was also initiated when the adjacent droplets contained single E. coli transformed with the OdDHL synthase gene, LasI, representing a simple quorum sensing circuit between two droplets.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
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Técnicas Analíticas Microfluídicas
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Escherichia coli
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Percepção de Quorum
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Análise de Célula Única
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article