Converting stem cells to dendritic cells by agonist antibodies from unbiased morphogenic selections.
Proc Natl Acad Sci U S A
; 110(37): 14966-71, 2013 Sep 10.
Article
em En
| MEDLINE
| ID: mdl-23980154
ABSTRACT
When combinatorial antibody libraries are rendered infectious for eukaryotic cells, the integrated antibody genotype and cellular phenotype become permanently linked and each cell becomes a selection system unto itself. These systems should be ideal for the identification of proteins and pathways that regulate differentiation so long as selection systems can be devised. Here we use a selection system based on the ability of secreted antibodies to alter the morphology of colonies expressing them when grown in soft agar. Importantly, this approach is different from all previous studies in that it used a pure discovery format where unbiased libraries that were not preselected against any known protein were used as probes. As such, the strategy is analogous to classical forward genetic approaches except that it operates directly at the protein level. This approach led to the identification of integrin-binding agonist antibodies that efficiently converted human stem cells to dendritic cells.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Células Dendríticas
/
Células-Tronco Hematopoéticas
/
Anticorpos
Limite:
Humans
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article