The aurora B kinase and the polycomb protein ring1B combine to regulate active promoters in quiescent lymphocytes.
Mol Cell
; 51(5): 647-61, 2013 Sep 12.
Article
em En
| MEDLINE
| ID: mdl-24034696
ABSTRACT
Reversible cellular quiescence is critical for developmental processes in metazoan organisms and is characterized by a reduction in cell size and transcriptional activity. We show that the Aurora B kinase and the polycomb protein Ring1B have essential roles in regulating transcriptionally active genes in quiescent lymphocytes. Ring1B and Aurora B bind to a wide range of active promoters in resting B and T cells. Conditional knockout of either protein results in reduced transcription and binding of RNA Pol II to promoter regions and decreased cell viability. Aurora B phosphorylates histone H3S28 at active promoters in resting B cells as well as inhibiting Ring1B-mediated ubiquitination of histone H2A and enhancing binding and activity of the USP16 deubiquitinase at transcribed genes. Our results identify a mechanism for regulating transcription in quiescent cells that has implications for epigenetic regulation of the choice between proliferation and quiescence.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Linfócitos B
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Linfócitos T
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Regiões Promotoras Genéticas
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Ubiquitina-Proteína Ligases
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Complexo Repressor Polycomb 1
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Aurora Quinase B
Limite:
Animals
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article