An efficient light-driven P450 BM3 biocatalyst.
J Am Chem Soc
; 135(39): 14484-7, 2013 Oct 02.
Article
em En
| MEDLINE
| ID: mdl-24040992
ABSTRACT
P450s are heme thiolate enzymes that catalyze the regio- and stereoselective functionalization of unactivated C-H bonds using molecular dioxygen and two electrons delivered by the reductase. We have developed hybrid P450 BM3 heme domains containing a covalently attached Ru(II) photosensitizer in order to circumvent the dependency on the reductase and perform P450 reactions upon visible light irradiation. A highly active hybrid enzyme with improved stability and a modified Ru(II) photosensitizer is able to catalyze the light-driven hydroxylation of lauric acid with total turnover numbers of 935 and initial reaction rate of 125 mol product/(mol enzyme/min).
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Base de dados:
MEDLINE
Assunto principal:
Rutênio
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Bacillus megaterium
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Proteínas de Bactérias
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NADPH-Ferri-Hemoproteína Redutase
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Fármacos Fotossensibilizantes
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Sistema Enzimático do Citocromo P-450
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Complexos de Coordenação
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article