Efficient foreign gene expression in planta using a plantago asiatica mosaic virus-based vector achieved by the strong RNA-silencing suppressor activity of TGBp1.
Arch Virol
; 159(5): 885-96, 2014 May.
Article
em En
| MEDLINE
| ID: mdl-24154949
ABSTRACT
Plant virus expression vectors provide a powerful tool for basic research as well as for practical applications. Here, we report the construction of an expression vector based on plantago asiatica mosaic virus (PlAMV), a member of the genus Potexvirus. Modification of a vector to enhance the expression of a foreign gene, combined with the use of the foot-and-mouth disease virus 2A peptide, allowed efficient expression of the foreign gene in two model plant species, Arabidopsis thaliana and Nicotiana benthamiana. Comparison with the widely used potato virus X (PVX) vector demonstrated that the PlAMV vector retains an inserted foreign gene for a longer period than PVX. Moreover, our results showed that the GFP expression construct PlAMV-GFP exhibits stronger RNA silencing suppression activity than PVX-GFP, which is likely to contribute to the stability of the PlAMV vector.
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Base de dados:
MEDLINE
Assunto principal:
Nicotiana
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Proteínas Virais
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Regulação Viral da Expressão Gênica
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Arabidopsis
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Potexvirus
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article