Sample prefractionation with liquid isoelectric focusing enables in depth microbial metaproteome analysis of mesophilic and thermophilic biogas plants.

Kohrs, F; Heyer, R; Magnussen, A; Benndorf, D; Muth, T; Behne, A; Rapp, E; Kausmann, R; Heiermann, M; Klocke, M; Reichl, U

Kohrs, F; Heyer, R; Magnussen, A; Benndorf, D; Muth, T; Behne, A; Rapp, E; Kausmann, R; Heiermann, M; Klocke, M; Reichl, U.

Afiliação

Kohrs F; Otto von Guericke University, Bioprocess Engineering, Universitätsplatz 2, 39106 Magdeburg, Germany.
Heyer R; Otto von Guericke University, Bioprocess Engineering, Universitätsplatz 2, 39106 Magdeburg, Germany.
Magnussen A; Otto von Guericke University, Bioprocess Engineering, Universitätsplatz 2, 39106 Magdeburg, Germany.
Benndorf D; Otto von Guericke University, Bioprocess Engineering, Universitätsplatz 2, 39106 Magdeburg, Germany. Electronic address: benndorf@mpi-magdeburg.mpg.de.
Muth T; Max Planck Institute for Dynamics of Complex Technical Systems, Bioprocess Engineering, Sandtorstraße 1, 39106 Magdeburg, Germany.
Behne A; Otto von Guericke University, Bioprocess Engineering, Universitätsplatz 2, 39106 Magdeburg, Germany.
Rapp E; Max Planck Institute for Dynamics of Complex Technical Systems, Bioprocess Engineering, Sandtorstraße 1, 39106 Magdeburg, Germany.
Kausmann R; Leibniz Institute for Agricultural Engineering Potsdam-Bornim, Technology Assessment and Substance Cycles, Max-Eyth-Allee 100, 14469 Potsdam, Germany.
Heiermann M; Leibniz Institute for Agricultural Engineering Potsdam-Bornim, Technology Assessment and Substance Cycles, Max-Eyth-Allee 100, 14469 Potsdam, Germany.
Klocke M; Leibniz Institute for Agricultural Engineering Potsdam-Bornim, Bioengineering, Max-Eyth-Allee 100, 14469 Potsdam, Germany.
Reichl U; Otto von Guericke University, Bioprocess Engineering, Universitätsplatz 2, 39106 Magdeburg, Germany; Max Planck Institute for Dynamics of Complex Technical Systems, Bioprocess Engineering, Sandtorstraße 1, 39106 Magdeburg, Germany.

Anaerobe ; 29: 59-67, 2014 Oct.

Article em En | MEDLINE | ID: mdl-24309213

Assuntos

Proteínas Arqueais/isolamento & purificação; Proteínas de Bactérias/isolamento & purificação; Proteínas Fúngicas/isolamento & purificação; Metano/biossíntese; Methanomicrobiales/metabolismo; Methanosarcinales/metabolismo; Proteoma/análise; Biocombustíveis; Reatores Biológicos; Eletroforese em Gel de Poliacrilamida/métodos; Focalização Isoelétrica/métodos; Metagenoma; Methanomicrobiales/química; Methanomicrobiales/genética; Methanosarcinales/química; Methanosarcinales/genética; Consórcios Microbianos/fisiologia; Plantas/metabolismo; Proteólise; Espectrometria de Massas em Tandem; Temperatura; Resíduos

Palavras-chave

Anaerobic digestion; Community profiling; Fractionation; GeLC-MS/MS; KEGG pathway analysis; Liquid isoelectric focusing; Metaproteomics

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Proteínas Fúngicas / Methanomicrobiales / Methanosarcinales / Proteínas Arqueais / Proteoma / Metano Idioma: En Ano de publicação: 2014 Tipo de documento: Article

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