[Effect of sanshui baihu decoction on the proliferation of fibroblast-like synoviocytes and its secretion of IL-6 and IL-17].
Zhongguo Zhong Xi Yi Jie He Za Zhi
; 33(10): 1385-8, 2013 Oct.
Article
em Zh
| MEDLINE
| ID: mdl-24432685
ABSTRACT
OBJECTIVE:
To observe the effect of Sanshui Baihu Decoction (SBD) containing serum on the proliferation of in vitro cultured fibroblast-like synoviocytes (FLS) derived from rheumatoid arthritis (RA) and osteoarthritis (OA) and its secretion of interleukin-6 (IL-6) and IL-17, and to explore the pharmacological mechanism of SBD.METHODS:
The FLS obtained from cultured RA and OA patients' synovial tissue were cultured and passaged in vitro in a routine way. The cultured medium was changed to DMEM with 20% SBD containing serum and cultured for 72 h after cultured for 3 to 6 generations. The proliferation rate of FLS was detected by MTT assay. Levels of IL-6 and IL-17 in the supernatant were detected by ELISA. Leflunomide and saline containing serum were used as positive and negative control respectively.RESULTS:
SBD containing serum significantly inhibited the proliferation of RA-FLS and OA-FLS, and decreased the secretion of IL-17 in RA-FLS. Its inhibition efficiency of SBD was equivalent to that of Leflunomide. No obvious inhibition on the secretion of IL-6 in RA-FLS was observed. It had no significant effect on the secretion of IL-17 and IL-6 in OA-FLS.CONCLUSION:
SBD could inhibit the proliferation of FLS and the secretion of IL-17 in RA-FLS, which might be one of its pharmacological mechanisms for treating RA.
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Base de dados:
MEDLINE
Assunto principal:
Artrite Reumatoide
/
Medicamentos de Ervas Chinesas
/
Proliferação de Células
/
Fibroblastos
Limite:
Animals
/
Humans
/
Male
Idioma:
Zh
Ano de publicação:
2013
Tipo de documento:
Article