Fabrication and neuron cytocompatibility of iron oxide nanoparticles coated with silk-fibroin peptides.
Colloids Surf B Biointerfaces
; 116: 465-71, 2014 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-24552663
ABSTRACT
Neuro-cytotoxicity of iron oxide nanoparticles (NPs) need to be considered. In this paper, magnetic Fe3O4 NPs are synthesized via a bio-mineralization process using silk fibroin (SF) as template. The prepared NPs with SF (SF-NPs) have more uniform grain size, better stability in weakly alkaline solution and higher saturation magnetization (about 82 emu/g) than pure NPs. After exposure to different concentration of SF-NPs (6.25-100 µg/mL), the reactive oxygen species generation in PC12 cells reduced compared with pure NPs. 1-5d treatment with SF-NPs did not destroy cell membrane and cyto-skeleton, and could improve the neurites extension in a dose-dependent manner at lower concentration (6.25-50 µg/mL), because SF peptide coating could delay the release of iron ions and the increase of surface crystal defects of NPs. Intact mitochondria in a neurite indicate the extension activity of neurites of cells treated with SF-NPs. Their high magnetic property and good neural-cytocompatibility provide the possibility of SF-NPs to be used as the contrast agents in brain MRI or the carrier of neural therapeutic drugs.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
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Materiais Biocompatíveis
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Seda
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Nanopartículas de Magnetita
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Fibroínas
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Neurônios
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article