Transporter assays and assay ontologies: useful tools for drug discovery.
Drug Discov Today Technol
; 12: e47-54, 2014 Jun.
Article
em En
| MEDLINE
| ID: mdl-25027375
ABSTRACT
Transport proteins represent an eminent class of drug targets and ADMET (absorption, distribution, metabolism, excretion, toxicity) associated genes. There exists a large number of distinct activity assays for transport proteins, depending on not only the measurement needed (e.g. transport activity, strength of ligandprotein interaction), but also due to heterogeneous assay setups used by different research groups. Efforts to systematically organize this (divergent) bioassay data have large potential impact in Public-Private partnership and conventional commercial drug discovery. In this short review, we highlight some of the frequently used high-throughput assays for transport proteins, and we discuss emerging assay ontologies and their application to this field. Focusing on human P-glycoprotein (Multidrug resistance protein 1; gene name ABCB1, MDR1), we exemplify how annotation of bioassay data per target class could improve and add to existing ontologies, and we propose to include an additional layer of metadata supporting data fusion across different bioassays.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Membrana Transportadoras
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Descoberta de Drogas
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Ensaios de Triagem em Larga Escala
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Ontologias Biológicas
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article