Single-cell genome-wide bisulfite sequencing for assessing epigenetic heterogeneity.
Nat Methods
; 11(8): 817-820, 2014 Aug.
Article
em En
| MEDLINE
| ID: mdl-25042786
ABSTRACT
We report a single-cell bisulfite sequencing (scBS-seq) method that can be used to accurately measure DNA methylation at up to 48.4% of CpG sites. Embryonic stem cells grown in serum or in 2i medium displayed epigenetic heterogeneity, with '2i-like' cells present in serum culture. Integration of 12 individual mouse oocyte datasets largely recapitulated the whole DNA methylome, which makes scBS-seq a versatile tool to explore DNA methylation in rare cells and heterogeneous populations.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Sulfitos
/
Genoma
/
Epigênese Genética
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article