Small intestinal stem cell identity is maintained with functional Paneth cells in heterotopically grafted epithelium onto the colon.
Genes Dev
; 28(16): 1752-7, 2014 Aug 15.
Article
em En
| MEDLINE
| ID: mdl-25128495
ABSTRACT
To develop stem cell therapy for small intestinal (SI) diseases, it is essential to determine whether SI stem cells in culture retain their tissue regeneration capabilities. By using a heterotopic transplantation approach, we show that cultured murine SI epithelial organoids are able to reconstitute self-renewing epithelia in the colon. When stably integrated, the SI-derived grafts show many features unique only to the SI but distinct from the colonic epithelium. Our study provides evidence that cultured adult SI stem cells could be a source for cell therapy of intestinal diseases, maintaining their identity along the gastrointestinal tract through an epithelium-intrinsic mechanism.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco
/
Colo
/
Celulas de Paneth
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Células Epiteliais
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Intestino Delgado
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article