Crystallization and preliminary X-ray diffraction analysis of a novel sphingomyelinase D from Loxosceles gaucho venom.
Acta Crystallogr F Struct Biol Commun
; 70(Pt 10): 1418-20, 2014 Oct.
Article
em En
| MEDLINE
| ID: mdl-25286953
ABSTRACT
Brown spider envenomation results in dermonecrosis, intravascular coagulation, haemolysis and renal failure, mainly owing to the action of sphingomyelinases D (SMases D), which catalyze the hydrolysis of sphingomyelin to produce ceramide 1-phosphate and choline or the hydrolysis of lysophosphatidylcholine to produce lysophosphatidic acid. Here, the heterologous expression, purification, crystallization and preliminary X-ray diffraction analysis of LgRec1, a novel SMase D from Loxosceles gaucho venom, are reported. The crystals belonged to space group P21212, with unit-cell parameters a = 52.98, b = 62.27, c = 84.84â
Å and diffracted to a maximum resolution of 2.6â
Å.
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Base de dados:
MEDLINE
Assunto principal:
Venenos de Aranha
/
Diester Fosfórico Hidrolases
/
Proteínas de Artrópodes
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article