Cas9-based genome editing in zebrafish.

Gonzales, Andrew P W; Yeh, Jing-Ruey Joanna

Gonzales, Andrew P W; Yeh, Jing-Ruey Joanna.

Afiliação

Gonzales AP; Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, Massachusetts, USA; Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA.
Yeh JR; Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, Massachusetts, USA; Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA. Electronic address: jyeh1@mgh.harvard.edu.

Methods Enzymol ; 546: 377-413, 2014.

Article em En | MEDLINE | ID: mdl-25398350

Assuntos

Sistemas CRISPR-Cas; Marcação de Genes/métodos; Peixe-Zebra/genética; Animais; Engenharia Genética/métodos; Genoma; Mutação INDEL; RNA Guia de Cinetoplastídeos/genética

Palavras-chave

CRISPR; Cas9; Chromosomal conversion; Chromosomal deletion; Gene-editing; Genome engineering; Homology-directed repair; Knock-in; Knock-out; Targeted integration; Targeted mutagenesis; Zebrafish

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Peixe-Zebra / Marcação de Genes / Sistemas CRISPR-Cas Limite: Animals Idioma: En Ano de publicação: 2014 Tipo de documento: Article

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