The N-N non-covalent domain of the nucleocapsid protein of type 2 porcine reproductive and respiratory syndrome virus enhances induction of IL-10 expression.
J Gen Virol
; 96(Pt 6): 1276-1286, 2015 Jun.
Article
em En
| MEDLINE
| ID: mdl-25614594
ABSTRACT
Porcine reproductive and respiratory syndrome virus (PRRSV) usually establishes a prolonged infection and causes an immunosuppressive state. It has been proposed that IL-10 plays an important role in PRRSV-induced immunosuppression. However, this mechanism has not been completely elucidated. In this study, we found that transfection of 3D4/2 macrophages with the N protein gene of type 2 PRRSV significantly upregulated IL-10 expression at the transcriptional level. Moreover, alanine substitution mutation analysis revealed that the N protein residues 33-37, 65-68 and 112-123 were related to the upregulation of IL-10 promoter activity. Recombinant PRRSV with mutations at residues 33-37 in the N protein (rQ33-5A and rS36A) recovered from corresponding infectious cDNA clones and induced significantly lower levels of IL-10 production in infected monocyte-derived dendritic cells, as compared with their revertants rQ33-5A(R) and rS36A(R), and the wild-type recombinant PRRSV strain rNT/wt. These data indicate that the type 2 PRRSV N protein plays an important role in IL-10 induction and the N-N non-covalent domain is associated with this activity.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Interleucina-10
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Vírus da Síndrome Respiratória e Reprodutiva Suína
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Proteínas do Nucleocapsídeo
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Interações Hospedeiro-Patógeno
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Evasão da Resposta Imune
Limite:
Animals
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article