Determination of Singlet Oxygen and Electron Transfer Mediated Mechanisms of Photosensitized Protein Damage by Phosphorus(V)porphyrins.
Chem Res Toxicol
; 28(2): 262-7, 2015 Feb 16.
Article
em En
| MEDLINE
| ID: mdl-25616052
ABSTRACT
The mechanism of photosensitized protein damage byphosphorus(V) tetraphenylporphyrin derivatives (P(V)TPPs) wasquantitatively clarified. P(V)TPPs bound to human serum albumin(HSA), a water-soluble protein, and damaged its tryptophan residueduring photoirradiation. P(V)TPPs photosensitized singlet oxygen ((1)O(2))generation, and the contribution of (1)O(2) to HSA damage was confirmedby the inhibitory effect of sodium azide, a (1)O(2) quencher. However,sodium azide could not completely inhibit HSA damage, suggesting thecontribution of an electron transfer mechanism to HSA damage. Thedecrement in the fluorescence lifetime of P(V)TPPs by HSA supportedthe electron transfer mechanism. The contribution of these processes could be determined by the kinetic analysis of the effect ofsodium azide on the photosensitized protein damage by P(V)TPPs.
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Base de dados:
MEDLINE
Assunto principal:
Fósforo
/
Porfirinas
/
Albumina Sérica
/
Oxigênio Singlete
/
Processos Fotoquímicos
Limite:
Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article