Expression profiling. Combinatorial labeling of single cells for gene expression cytometry.
Science
; 347(6222): 1258367, 2015 Feb 06.
Article
em En
| MEDLINE
| ID: mdl-25657253
ABSTRACT
We present a technically simple approach for gene expression cytometry combining next-generation sequencing with stochastic barcoding of single cells. A combinatorial library of beads bearing cell- and molecular-barcoding capture probes is used to uniquely label transcripts and reconstruct the digital gene expression profile of thousands of individual cells in a single experiment without the need for robotics or automation. We applied the technology to dissect the human hematopoietic system and to characterize heterogeneous response to in vitro stimulation. High sensitivity is demonstrated by detection of low-abundance transcripts and rare cells. Under current implementation, the technique can analyze a few thousand cells simultaneously and can readily scale to 10,000s or 100,000s of cells.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
RNA Mensageiro
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Técnicas de Química Combinatória
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Perfilação da Expressão Gênica
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Análise de Célula Única
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Código de Barras de DNA Taxonômico
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Citometria de Fluxo
Tipo de estudo:
Evaluation_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article