The Spectrin cytoskeleton regulates the Hippo signalling pathway.
EMBO J
; 34(7): 940-54, 2015 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-25712476
ABSTRACT
The Spectrin cytoskeleton is known to be polarised in epithelial cells, yet its role remains poorly understood. Here, we show that the Spectrin cytoskeleton controls Hippo signalling. In the developing Drosophila wing and eye, loss of apical Spectrins (alpha/beta-heavy dimers) produces tissue overgrowth and mis-regulation of Hippo target genes, similar to loss of Crumbs (Crb) or the FERM-domain protein Expanded (Ex). Apical beta-heavy Spectrin binds to Ex and co-localises with it at the apical membrane to antagonise Yki activity. Interestingly, in both the ovarian follicular epithelium and intestinal epithelium of Drosophila, apical Spectrins and Crb are dispensable for repression of Yki, while basolateral Spectrins (alpha/beta dimers) are essential. Finally, the Spectrin cytoskeleton is required to regulate the localisation of the Hippo pathway effector YAP in response to cell density human epithelial cells. Our findings identify both apical and basolateral Spectrins as regulators of Hippo signalling and suggest Spectrins as potential mechanosensors.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Citoesqueleto
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Espectrina
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Proteínas Serina-Treonina Quinases
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Proteínas de Drosophila
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Mecanotransdução Celular
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Peptídeos e Proteínas de Sinalização Intracelular
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Folículo Ovariano
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Female
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Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article