Your browser doesn't support javascript.
loading
Identification, characterization and full-length sequence analysis of a novel dsRNA virus isolated from the arboreal ant Camponotus yamaokai.
Koyama, Satoshi; Urayama, Syun-Ichi; Ohmatsu, Tsutomu; Sassa, Yukiko; Sakai, Chihiro; Takata, Mamoru; Hayashi, Shinya; Nagai, Makoto; Furuya, Tetsuya; Moriyama, Hiromitsu; Satoh, Toshiyuki; Ono, Shin-Ichi; Mizutani, Testuya.
Afiliação
  • Koyama S; 1Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, Japan.
  • Urayama S; 1Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, Japan.
  • Ohmatsu T; 2Research and Education Center for Prevention of Global Infectious Disease of Animals, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, Japan.
  • Sassa Y; 1Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, Japan.
  • Sakai C; 3Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, Japan.
  • Takata M; 1Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, Japan.
  • Hayashi S; 1Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, Japan.
  • Nagai M; 1Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, Japan.
  • Furuya T; 1Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, Japan.
  • Moriyama H; 1Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, Japan.
  • Satoh T; 1Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, Japan.
  • Ono S; 4Department of Fisheries, School of Marine Science and Technology, Tokai University, 3-20-1 Orido, Shimizu, Shizuoka, Japan.
  • Mizutani T; 2Research and Education Center for Prevention of Global Infectious Disease of Animals, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, Japan.
J Gen Virol ; 96(Pt 7): 1930-7, 2015 Jul.
Article em En | MEDLINE | ID: mdl-25787938
ABSTRACT
A novel dsRNA virus was identified from the arboreal ant Camponotus yamaokai. The complete nucleotide sequence analysis of the virus revealed that the virus consisted of 5704 bp with two ORFs. ORF1 (3084 nt) encoded a putative capsid protein. ORF2 (1977 nt) encoded a viral RNA-dependent RNA polymerase (RdRp). ORF2 could be translated as a fusion with the ORF1 product by a - 1 frameshift in the overlapping ORF1. Phylogenetic analyses based on the RdRp revealed that the virus from C. yamaokai was most likely a novel totivirus, but it was not closely related to the previously known totiviruses in arthropods. Transmission electron microscopy revealed isometric virus particles of ~30 nm diameter in the cytoplasm, which was consistent with the characteristics of the family Totiviridae. The virus was detected by reverse transcription-PCR in all caste members and developmental stages of ants, including eggs, larvae, pupae, adult workers, alates (male and female) and queens. To our knowledge, this is the first report of a member of the family Totiviridae in a hymenopteran; the virus was designated Camponotus yamaokai virus.
Assuntos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Formigas / Vírus de RNA / RNA de Cadeia Dupla / RNA Viral / Genoma Viral Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2015 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Formigas / Vírus de RNA / RNA de Cadeia Dupla / RNA Viral / Genoma Viral Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2015 Tipo de documento: Article