Synthesis of N2, N2, 7-trimethylguanosine cap derivatives.
Nucleic Acids Res
; 17(22): 8979-89, 1989 Nov 25.
Article
em En
| MEDLINE
| ID: mdl-2587250
ABSTRACT
Several derivatives of N2,N2-7-trimethylguanosine (m3(2,2,7G)-cap, which was found at the 5' ends of small nuclear RNAs, were synthesized by use of S-phenyl N2,N2,7-trimethylguanosine 5'-phosphorothioate (PhSpm3(2,2,7)G) as a key intermediate. This compound was activated by iodine in the presence of phosphoric acid and diphosphoric acid to give N2,N2,7-trimethylguanosine-5'-diphosphate (ppm3(2,2,7)G) and 5'-triphosphate (ppm3(2,2,7)G), respectively. Similar reactions of PhSpm3(2,2,7)G with ADP and GDP gave capped dinucleoside triphosphates, m3(2,2,7)G5'pppA and m3(2,2,7)G5'pppG, respectively.
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Base de dados:
MEDLINE
Assunto principal:
Capuzes de RNA
Idioma:
En
Ano de publicação:
1989
Tipo de documento:
Article