Crystal structure of Cry51Aa1: A potential novel insecticidal aerolysin-type ß-pore-forming toxin from Bacillus thuringiensis.
Biochem Biophys Res Commun
; 462(3): 184-9, 2015 Jul 03.
Article
em En
| MEDLINE
| ID: mdl-25957471
ABSTRACT
The structures of several Bacillus thuringiensis (Bt) insecticidal crystal proteins have been determined by crystallographic methods and a close relationship has been explicated between specific toxicities and conserved three-dimensional architectures. In this study, as a representative of the coleopteran- and hemipteran-specific Cry51A group, the complete structure of Cry51Aa1 protoxin has been determined by X-ray crystallography at 1.65 Å resolution. This is the first report of a coleopteran-active Bt insecticidal toxin with high structural similarity to the aerolysin-type ß-pore forming toxins (ß-PFTs). Moreover, study of featured residues and structural elements reveal their possible roles in receptor binding and pore formation events. This study provides new insights into the action of aerolysin-type ß-PFTs from a structural perspective, and could be useful for the control of coleopteran and hemipteran insect pests in agricultures.
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MEDLINE
Assunto principal:
Bacillus thuringiensis
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Proteínas de Bactérias
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Toxinas Bacterianas
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Endotoxinas
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Proteínas Citotóxicas Formadoras de Poros
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Proteínas Hemolisinas
Limite:
Animals
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article