Commensal bacteria direct selective cargo sorting to promote symbiosis.
Nat Immunol
; 16(9): 918-26, 2015 Sep.
Article
em En
| MEDLINE
| ID: mdl-26237551
ABSTRACT
Mucosal immunity protects a host from intestinal inflammation and infection and is profoundly influenced by symbiotic bacteria. Here we report that in mice symbiotic bacteria directed selective cargo sorting in Paneth cells to promote symbiosis through Nod2, a cytosolic bacterial sensor, and the multifunctional protein kinase LRRK2, both encoded by inflammatory bowel disease (IBD)-associated genes. Commensals recruited Nod2 onto lysozyme-containing dense core vesicles (DCVs), which was required for DCV localization of LRRK2 and a small GTPase, Rab2a. Deficiency of Nod2, LRRK2 or Rab2a or depletion of commensals resulted in lysosomal degradation of lysozyme. Thus, commensal bacteria and host factors orchestrate the lysozyme-sorting process to protect the host from enteric infection, implicating Paneth cell dysfunction in IBD pathogenesis.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Simbiose
/
Doenças Inflamatórias Intestinais
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Proteínas Serina-Treonina Quinases
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Imunidade nas Mucosas
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Celulas de Paneth
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Enterocolite
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Intestinos
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Listeriose
Limite:
Animals
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article