Synthesis and validation of novel cholesterol-based fluorescent lipids designed to observe the cellular trafficking of cationic liposomes.
Bioorg Med Chem Lett
; 25(18): 3893-6, 2015 Sep 15.
Article
em En
| MEDLINE
| ID: mdl-26243368
ABSTRACT
Cholesterol-based fluorescent lipids with ether linker were synthesized using NBD (Chol-E-NBD) or Rhodamine B (Chol-E-Rh), and the usefulnesses as fluorescent probes for tracing cholesterol-based liposomes were validated. The fluorescent intensities of liposomes containing these modified lipids were measured and observed under a microscope. Neither compound interfered with the expression of GFP plasmid, and live cell images were obtained without interferences. Changes in the fluorescent intensity of liposomes containing Chol-E-NBD were followed by flow cytometry for up to 24h. These fluorescent lipids could be useful probes for trafficking of cationic liposome-mediated gene delivery.
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Base de dados:
MEDLINE
Assunto principal:
Colesterol
/
Corantes Fluorescentes
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Lipídeos
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Lipossomos
Limite:
Animals
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article