Dynamic enhancer-gene body contacts during transcription elongation.
Genes Dev
; 29(19): 1992-7, 2015 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-26443845
ABSTRACT
Enhancers govern transcription through multiple mechanisms, including the regulation of elongation by RNA polymerase II (RNAPII). We characterized the dynamics of looped enhancer contacts during synchronous transcription elongation. We found that many distal enhancers form stable contacts with their target promoters during the entire interval of elongation. Notably, we detected additional dynamic enhancer contacts throughout the gene bodies that track with elongating RNAPII and the leading edge of RNA synthesis. These results support a model in which the gene body changes its position relative to a stable enhancer-promoter complex, which has broad ramifications for enhancer function and architectural models of transcriptional elongation.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Regulação da Expressão Gênica
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Elementos Facilitadores Genéticos
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Regiões Promotoras Genéticas
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Globinas beta
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Elongação da Transcrição Genética
Limite:
Animals
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article