EFFECT OF SOLID MEDIUM DURING COOLED STORAGE ON STALLION SPERM PARAMETERS.
Cryo Letters
; 36(5): 313-7, 2015.
Article
em En
| MEDLINE
| ID: mdl-26574678
ABSTRACT
BACKGROUND:
Solid storage medium prevents cellular sedimentation, reduces metabolic demand via limiting movement, and avoids the modification of an extender composition in the sedimentary microenvironment. It has been proven to prolong spermatozoa viability in mammalians.OBJECTIVE:
This experiment aims to evaluate the effect of cool storage in solid phase extender on stallion sperms. MATERIALS ANDMETHODS:
Semen was collected from 10 Crioulo stallions (n=30) and submitted to treatments control group (semen extender) and groups with gelatin addition in different concentrations (semen extender + 1%, 2% and 3%). Seminal analyses included motility, mitochondrial functionality, plasma membrane integrity, DNA and acrosome at 0; 24; 48 and 72 hours during cooled storage at 5 degree C.RESULTS:
Motility, mitochondrial functionality, plasma membrane and acrosome integrity declined during storage time, with no statistical difference between treatments. DNA integrity did not significantly change during storage period.CONCLUSION:
Solid medium was not harmful and did not improved stallion sperm parameters during cooled storage.
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Base de dados:
MEDLINE
Assunto principal:
Preservação do Sêmen
/
Espermatozoides
/
Criopreservação
/
Cavalos
Limite:
Animals
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article