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Treatment with intranasal iloprost reduces disease manifestations in a murine model of previously established COPD.
Lammi, Matthew R; Ghonim, Mohamed A; Pyakurel, Kusma; Naura, Amarjit S; Ibba, Salome V; Davis, Christian J; Okpechi, Samuel C; Happel, Kyle I; deBoisblanc, Bennett P; Shellito, Judd; Boulares, A Hamid.
Afiliação
  • Lammi MR; Louisiana State University Health Sciences Center, New Orleans, Lousiana; mlammi@lsuhsc.edu.
  • Ghonim MA; Louisiana State University Health Sciences Center, New Orleans, Lousiana; Stanley Scott Cancer Center, New Orleans, Louisiana; and.
  • Pyakurel K; Louisiana State University Health Sciences Center, New Orleans, Lousiana; Stanley Scott Cancer Center, New Orleans, Louisiana; and.
  • Naura AS; Panjab University, Chandigarh, India.
  • Ibba SV; Louisiana State University Health Sciences Center, New Orleans, Lousiana; Stanley Scott Cancer Center, New Orleans, Louisiana; and.
  • Davis CJ; Louisiana State University Health Sciences Center, New Orleans, Lousiana; Stanley Scott Cancer Center, New Orleans, Louisiana; and.
  • Okpechi SC; Louisiana State University Health Sciences Center, New Orleans, Lousiana; Stanley Scott Cancer Center, New Orleans, Louisiana; and.
  • Happel KI; Louisiana State University Health Sciences Center, New Orleans, Lousiana;
  • deBoisblanc BP; Louisiana State University Health Sciences Center, New Orleans, Lousiana;
  • Shellito J; Louisiana State University Health Sciences Center, New Orleans, Lousiana;
  • Boulares AH; Louisiana State University Health Sciences Center, New Orleans, Lousiana; Stanley Scott Cancer Center, New Orleans, Louisiana; and.
Am J Physiol Lung Cell Mol Physiol ; 310(7): L630-8, 2016 04 01.
Article em En | MEDLINE | ID: mdl-26851260
Pulmonary endothelial prostacyclin appears to be involved in the pathogenesis of chronic obstructive pulmonary disease (COPD). The effect of treatment with a prostacyclin analog in animal models of previously established COPD is unknown. We evaluated the short- and long-term effect of iloprost on inflammation and airway hyperresponsiveness (AHR) in a murine model of COPD. Nineteen mice were exposed to LPS/elastase, followed by either three doses of intranasal iloprost or saline. In the long-term treatment experiment, 18 mice were exposed to LPS/elastase and then received 6 wk of iloprost or were left untreated as controls. In the short-term experiment, iloprost did not change AHR but significantly reduced serum IL-5 and IFN-γ. Long-term treatment with iloprost for both 2 and 6 wk significantly improved AHR. After 6 wk of iloprost, there was a reduction in bronchoalveolar lavage (BALF) neutrophils, serum IL-1ß (30.0 ± 9.2 vs. 64.8 ± 7.4 pg/ml, P = 0.045), IL-2 (36.5 ± 10.6 vs. 83.8 ± 0.4 pg/ml, P = 0.01), IL-10 (75.7 ± 9.3 vs. 96.5 ± 3.5 pg/ml, P = 0.02), and nitrite (15.1 ± 5.4 vs. 30.5 ± 10.7 µmol, P = 0.01). Smooth muscle actin (SMA) in the lung homogenate was also significantly reduced after iloprost treatment (P = 0.02), and SMA thickness was reduced in the small and medium blood vessels after iloprost (P < 0.001). In summary, short- and long-term treatment with intranasal iloprost significantly reduced systemic inflammation in an LPS/elastase COPD model. Long-term iloprost treatment also reduced AHR, serum nitrite, SMA, and BALF neutrophilia. These data encourage future investigations of prostanoid therapy as a novel treatment for COPD patients.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Iloprosta / Doença Pulmonar Obstrutiva Crônica / Anti-Inflamatórios Limite: Animals Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Iloprosta / Doença Pulmonar Obstrutiva Crônica / Anti-Inflamatórios Limite: Animals Idioma: En Ano de publicação: 2016 Tipo de documento: Article