Phase separation of signaling molecules promotes T cell receptor signal transduction.
Science
; 352(6285): 595-9, 2016 Apr 29.
Article
em En
| MEDLINE
| ID: mdl-27056844
ABSTRACT
Activation of various cell surface receptors triggers the reorganization of downstream signaling molecules into micrometer- or submicrometer-sized clusters. However, the functional consequences of such clustering have been unclear. We biochemically reconstituted a 12-component signaling pathway on model membranes, beginning with T cell receptor (TCR) activation and ending with actin assembly. When TCR phosphorylation was triggered, downstream signaling proteins spontaneously separated into liquid-like clusters that promoted signaling outputs both in vitro and in human Jurkat T cells. Reconstituted clusters were enriched in kinases but excluded phosphatases and enhanced actin filament assembly by recruiting and organizing actin regulators. These results demonstrate that protein phase separation can create a distinct physical and biochemical compartment that facilitates signaling.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Receptores de Antígenos de Linfócitos T
/
Linfócitos T
/
Actinas
/
Proteínas Adaptadoras de Transdução de Sinal
/
Proteínas de Membrana
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article