microRNA signatures in peripheral blood fail to detect acute cellular rejection after liver transplantation.

Raschzok, Nathanael; Reutzel-Selke, Anja; Schmuck, Rosa B; Tannus, Laura M; Morgul, Mehmet H; Dietel, Corinna; Leder, Annekatrin; Struecker, Benjamin; Lippert, Steffen; Sallmon, Hannes; Schmelzle, Moritz; Bartels, Michael; Jonas, Sven; Pratschke, Johann; Sauer, Igor M

Raschzok, Nathanael; Reutzel-Selke, Anja; Schmuck, Rosa B; Tannus, Laura M; Morgul, Mehmet H; Dietel, Corinna; Leder, Annekatrin; Struecker, Benjamin; Lippert, Steffen; Sallmon, Hannes; Schmelzle, Moritz; Bartels, Michael; Jonas, Sven; Pratschke, Johann; Sauer, Igor M.

Afiliação

Raschzok N; a Department of General, Visceral, and Transplantation Surgery, Experimental Surgery and Regenerative Medicine , Charité - Universitätsmedizin Berlin, Campus Virchow Klinikum , Berlin , Germany.
Reutzel-Selke A; a Department of General, Visceral, and Transplantation Surgery, Experimental Surgery and Regenerative Medicine , Charité - Universitätsmedizin Berlin, Campus Virchow Klinikum , Berlin , Germany.
Schmuck RB; a Department of General, Visceral, and Transplantation Surgery, Experimental Surgery and Regenerative Medicine , Charité - Universitätsmedizin Berlin, Campus Virchow Klinikum , Berlin , Germany.
Tannus LM; a Department of General, Visceral, and Transplantation Surgery, Experimental Surgery and Regenerative Medicine , Charité - Universitätsmedizin Berlin, Campus Virchow Klinikum , Berlin , Germany.
Morgul MH; a Department of General, Visceral, and Transplantation Surgery, Experimental Surgery and Regenerative Medicine , Charité - Universitätsmedizin Berlin, Campus Virchow Klinikum , Berlin , Germany.
Dietel C; b Department of Visceral, Transplantation, Thoracic, and Vascular Surgery , University of Leipzig , Leipzig , Germany.
Leder A; b Department of Visceral, Transplantation, Thoracic, and Vascular Surgery , University of Leipzig , Leipzig , Germany.
Struecker B; a Department of General, Visceral, and Transplantation Surgery, Experimental Surgery and Regenerative Medicine , Charité - Universitätsmedizin Berlin, Campus Virchow Klinikum , Berlin , Germany.
Lippert S; a Department of General, Visceral, and Transplantation Surgery, Experimental Surgery and Regenerative Medicine , Charité - Universitätsmedizin Berlin, Campus Virchow Klinikum , Berlin , Germany.
Sallmon H; a Department of General, Visceral, and Transplantation Surgery, Experimental Surgery and Regenerative Medicine , Charité - Universitätsmedizin Berlin, Campus Virchow Klinikum , Berlin , Germany.
Schmelzle M; c Department of Neonatology , Charité - Universitätsmedizin Berlin , Germany.
Bartels M; a Department of General, Visceral, and Transplantation Surgery, Experimental Surgery and Regenerative Medicine , Charité - Universitätsmedizin Berlin, Campus Virchow Klinikum , Berlin , Germany.
Jonas S; d Department of Translational Centre for Regenerative Medicine , University of Leipzig , Leipzig , Germany.
Pratschke J; b Department of Visceral, Transplantation, Thoracic, and Vascular Surgery , University of Leipzig , Leipzig , Germany.
Sauer IM; e Centre Hépato-Biliaire , Hôpital Paul Brousse, Université Paris Sud , Villejuif , France.

Biomarkers ; 21(8): 699-707, 2016 Dec.

Article em En | MEDLINE | ID: mdl-27121640

ABSTRACT

ABSTRACT

OBJECTIVE:

We investigated whether microRNA signatures in whole blood samples are associated with acute cellular rejection (ACR) after liver transplantation. MATERIALS AND

METHODS:

Blood samples were collected using Paxgene technology and analyzed by microarrays and quantitative real-time polymerase chain reaction (qRT-PCR).

RESULTS:

microRNA signatures failed to distinguish between 19 patients with ACR and 16 controls. Let-7b-5p and let-7c were upregulated in a subgroup of patients with ACR during the 6th and 7th postoperative days but failed in an independent validation of 20 patients.