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The De-Etiolated 1 Homolog of Arabidopsis Modulates the ABA Signaling Pathway and ABA Biosynthesis in Rice.
Zang, Guangchao; Zou, Hanyan; Zhang, Yuchan; Xiang, Zheng; Huang, Junli; Luo, Li; Wang, Chunping; Lei, Kairong; Li, Xianyong; Song, Deming; Din, Ahmad Ud; Wang, Guixue.
Afiliação
  • Zang G; Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College of Chongqing University, Chongqing 400030, China (G.Z., H.Z., Z.X., J.H., L.L., A.U.D., G.W.);Institute of Life Science, Chongqing Medical University, Chongqing 400016, China
  • Zou H; Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College of Chongqing University, Chongqing 400030, China (G.Z., H.Z., Z.X., J.H., L.L., A.U.D., G.W.);Institute of Life Science, Chongqing Medical University, Chongqing 400016, China
  • Zhang Y; Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College of Chongqing University, Chongqing 400030, China (G.Z., H.Z., Z.X., J.H., L.L., A.U.D., G.W.);Institute of Life Science, Chongqing Medical University, Chongqing 400016, China
  • Xiang Z; Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College of Chongqing University, Chongqing 400030, China (G.Z., H.Z., Z.X., J.H., L.L., A.U.D., G.W.);Institute of Life Science, Chongqing Medical University, Chongqing 400016, China
  • Huang J; Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College of Chongqing University, Chongqing 400030, China (G.Z., H.Z., Z.X., J.H., L.L., A.U.D., G.W.);Institute of Life Science, Chongqing Medical University, Chongqing 400016, China
  • Luo L; Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College of Chongqing University, Chongqing 400030, China (G.Z., H.Z., Z.X., J.H., L.L., A.U.D., G.W.);Institute of Life Science, Chongqing Medical University, Chongqing 400016, China
  • Wang C; Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College of Chongqing University, Chongqing 400030, China (G.Z., H.Z., Z.X., J.H., L.L., A.U.D., G.W.);Institute of Life Science, Chongqing Medical University, Chongqing 400016, China
  • Lei K; Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College of Chongqing University, Chongqing 400030, China (G.Z., H.Z., Z.X., J.H., L.L., A.U.D., G.W.);Institute of Life Science, Chongqing Medical University, Chongqing 400016, China
  • Li X; Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College of Chongqing University, Chongqing 400030, China (G.Z., H.Z., Z.X., J.H., L.L., A.U.D., G.W.);Institute of Life Science, Chongqing Medical University, Chongqing 400016, China
  • Song D; Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College of Chongqing University, Chongqing 400030, China (G.Z., H.Z., Z.X., J.H., L.L., A.U.D., G.W.);Institute of Life Science, Chongqing Medical University, Chongqing 400016, China
  • Din AU; Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College of Chongqing University, Chongqing 400030, China (G.Z., H.Z., Z.X., J.H., L.L., A.U.D., G.W.);Institute of Life Science, Chongqing Medical University, Chongqing 400016, China
  • Wang G; Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College of Chongqing University, Chongqing 400030, China (G.Z., H.Z., Z.X., J.H., L.L., A.U.D., G.W.);Institute of Life Science, Chongqing Medical University, Chongqing 400016, China
Plant Physiol ; 171(2): 1259-76, 2016 06.
Article em En | MEDLINE | ID: mdl-27208292
DEETIOLATED1 (DET1) plays a critical role in developmental and environmental responses in many plants. To date, the functions of OsDET1 in rice (Oryza sativa) have been largely unknown. OsDET1 is an ortholog of Arabidopsis (Arabidopsis thaliana) DET1 Here, we found that OsDET1 is essential for maintaining normal rice development. The repression of OsDET1 had detrimental effects on plant development, and leaded to contradictory phenotypes related to abscisic acid (ABA) in OsDET1 interference (RNAi) plants. We found that OsDET1 is involved in modulating ABA signaling in rice. OsDET1 RNAi plants exhibited an ABA hypersensitivity phenotype. Using yeast two-hybrid (Y2H) and bimolecular fluorescence complementation assays, we determined that OsDET1 interacts physically with DAMAGED-SPECIFIC DNA-BINDING PROTEIN1 (OsDDB1) and CONSTITUTIVE PHOTOMORPHOGENIC10 (COP10); DET1- and DDB1-ASSOCIATED1 binds to the ABA receptors OsPYL5 and OsDDB1. We found that the degradation of OsPYL5 was delayed in OsDET1 RNAi plants. These findings suggest that OsDET1 deficiency disturbs the COP10-DET1-DDB1 complex, which is responsible for ABA receptor (OsPYL) degradation, eventually leading to ABA sensitivity in rice. Additionally, OsDET1 also modulated ABA biosynthesis, as ABA biosynthesis was inhibited in OsDET1 RNAi plants and promoted in OsDET1-overexpressing transgenic plants. In conclusion, our data suggest that OsDET1 plays an important role in maintaining normal development in rice and mediates the cross talk between ABA biosynthesis and ABA signaling pathways in rice.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Plantas / Oryza / Proteínas Nucleares / Transdução de Sinais / Homologia de Sequência de Aminoácidos / Ácido Abscísico / Proteínas de Arabidopsis / Estiolamento Idioma: En Ano de publicação: 2016 Tipo de documento: Article
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Plantas / Oryza / Proteínas Nucleares / Transdução de Sinais / Homologia de Sequência de Aminoácidos / Ácido Abscísico / Proteínas de Arabidopsis / Estiolamento Idioma: En Ano de publicação: 2016 Tipo de documento: Article