CRISPR/Cas9 for Human Genome Engineering and Disease Research.
Annu Rev Genomics Hum Genet
; 17: 131-54, 2016 08 31.
Article
em En
| MEDLINE
| ID: mdl-27216776
ABSTRACT
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) system, a versatile RNA-guided DNA targeting platform, has been revolutionizing our ability to modify, manipulate, and visualize the human genome, which greatly advances both biological research and therapeutics development. Here, we review the current development of CRISPR/Cas9 technologies for gene editing, transcription regulation, genome imaging, and epigenetic modification. We discuss the broad application of this system to the study of functional genomics, especially genome-wide genetic screening, and to therapeutics development, including establishing disease models, correcting defective genetic mutations, and treating diseases.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Terapia Genética
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Genoma Humano
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Sistemas CRISPR-Cas
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Edição de Genes
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article