Layered Double Hydroxide Nanotransporter for Molecule Delivery to Intact Plant Cells.
Sci Rep
; 6: 26738, 2016 05 25.
Article
em En
| MEDLINE
| ID: mdl-27221055
ABSTRACT
Here we report a powerful method that facilitates the transport of biologically active materials across the cell wall barrier in plant cells. Positively charged delaminated layered double hydroxide lactate nanosheets (LDH-lactate-NS) with a 0.5â2 nm thickness and 30â60 nm diameter exhibit a high adsorptive capacity for negatively charged biomolecules, including fluorescent dyes such as tetramethyl rhodamine isothiocyanate (TRITC), fluorescein isothiocyanate isomer I(FITC) and DNA molecules, forming neutral LDH-nanosheet conjugates. These neutral conjugates can shuttle the bound fluorescent dye into the cytosol of intact plant cell very efficiently. Furthermore, typical inhibitors of endocytosis and low temperature incubation did not prevent LDH-lactate-NS internalization, suggesting that LDH-lactate-NS penetrated the plasma membrane via non-endocytic pathways, which will widen the applicability to a variety of plant cells. Moreover, the absence of unwanted side effects in our cytological studies, and the nuclear localization of ssDNA-FITC suggest that nano-LDHs have potential application as a novel gene carrier to plants.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Nicotiana
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Portadores de Fármacos
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Nanopartículas
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Células Vegetais
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Lactatos
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article