Real-time intravital imaging of pH variation associated with osteoclast activity.
Nat Chem Biol
; 12(8): 579-85, 2016 08.
Article
em En
| MEDLINE
| ID: mdl-27272564
ABSTRACT
Intravital imaging by two-photon excitation microscopy (TPEM) has been widely used to visualize cell functions. However, small molecular probes (SMPs), commonly used for cell imaging, cannot be simply applied to intravital imaging because of the challenge of delivering them into target tissues, as well as their undesirable physicochemical properties for TPEM imaging. Here, we designed and developed a functional SMP with an active-targeting moiety, higher photostability, and a fluorescence switch and then imaged target cell activity by injecting the SMP into living mice. The combination of the rationally designed SMP with a fluorescent protein as a reporter of cell localization enabled quantitation of osteoclast activity and time-lapse imaging of its in vivo function associated with changes in cell deformation and membrane fluctuations. Real-time imaging revealed heterogenic behaviors of osteoclasts in vivo and provided insights into the mechanism of bone resorption.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Osteoclastos
/
Imagem Molecular
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Imagem com Lapso de Tempo
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Microscopia Intravital
Tipo de estudo:
Risk_factors_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article