From the Worm in a Bottle of Mezcal: iDNA Confirmation of a Leech Parasitizing the Antillean Manatee.
J Parasitol
; 102(5): 553-555, 2016 Oct.
Article
em En
| MEDLINE
| ID: mdl-27391275
ABSTRACT
Invertebrate-derived ingested DNA (iDNA) is quickly proving to be a valuable, non-invasive tool for monitoring vertebrate species of conservation concern. Using the DNA barcoding locus, we successfully identified both the blood-feeding leech Haementeria acuecueyetzin and its blood meal-the latter is shown to be derived from the Caribbean manatee, Trichechus manatus . DNA amplification was successful despite the fact that the specimen was fixed in Mezcal (a beverage distilled from agave). We report the first confirmed case of a leech feeding on a manatee, the first record of H. acuecueyetzin for the State of Chiapas and, to our knowledge, the first case of successful DNA amplification of a biological sample fixed in Mezcal other than the caterpillar "worms" more commonly found in that beverage.
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Base de dados:
MEDLINE
Assunto principal:
Trichechus
/
Agave
/
Código de Barras de DNA Taxonômico
/
Sucos de Frutas e Vegetais
/
Sanguessugas
Limite:
Animals
País/Região como assunto:
Caribe
/
Mexico
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article