Antibodies against HLA-DP recognize broadly expressed epitopes.
Hum Immunol
; 77(12): 1128-1139, 2016 Dec.
Article
em En
| MEDLINE
| ID: mdl-27664843
ABSTRACT
HLA matching and avoidance of pre-transplant donor-specific antibodies are important in selection of donors for solid organ transplant. Solid phase testing with single antigen beads allows resolution of antibody reactivity to the level of the allele. Single antigen bead testing results at a large transplant center were reviewed to identify selective reactivity patterns of anti-HLA antibodies. Many HLA-DP antibodies were identified in the context of other HLA antibodies, but some sera had antibodies against only HLA-DP. B cell flow crossmatch testing was positive for 2 out of 9 sera with HLA-DP antibodies. Many patterns of reactivity corresponded to epitopes in hypervariable regions C and F of DPB1, but some matched epitopes in other regions or DPA1. Through analysis of single antigen bead testing from a large number of patients, we report that anti-HLA-DP antibodies predominantly recognize broadly cross-reactive epitopes. The United Network for Organ Sharing has mandated HLA-DP typing on all deceased kidney donors, and HLA-DP epitopes should be considered as the major antigens for avoidance of pre-transplant donor-specific antibodies.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Teste de Histocompatibilidade
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Transplante de Rim
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Epitopos de Linfócito B
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Cadeias beta de HLA-DP
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Rejeição de Enxerto
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article