Development of a bacterial-based negative selection system for rapid screening of active single guide RNAs.

Nie, Yu; Cong, Peiqing; Liu, Xiaofeng; Wang, Min; Chen, Yaosheng; He, Zuyong

Nie, Yu; Cong, Peiqing; Liu, Xiaofeng; Wang, Min; Chen, Yaosheng; He, Zuyong.

Afiliação

Nie Y; Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510006, People's Republic of China.
Cong P; Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510006, People's Republic of China.
Liu X; Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510006, People's Republic of China.
Wang M; Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510006, People's Republic of China.
Chen Y; Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510006, People's Republic of China.
He Z; Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510006, People's Republic of China. zuyonghe@foxmail.com.

Biotechnol Lett ; 39(3): 351-358, 2017 Mar.

Article em En | MEDLINE | ID: mdl-27858321

Assuntos

Escherichia coli/genética; Testes Genéticos/métodos; RNA Guia de Cinetoplastídeos/genética; Animais; Sequência de Bases; Células HEK293; Humanos; Mutação INDEL/genética; Camundongos; Plasmídeos/genética; RNA Guia de Cinetoplastídeos/metabolismo; Sus scrofa

Palavras-chave

Bacterial-based negative selection system; CRISPR/Cas9; Cas nuclease; Single guide RNA; T7E1 assay

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Testes Genéticos / RNA Guia de Cinetoplastídeos / Escherichia coli Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2017 Tipo de documento: Article

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