Radioiodinated Capsids Facilitate In Vivo Non-Invasive Tracking of Adeno-Associated Gene Transfer Vectors.
Sci Rep
; 7: 39594, 2017 01 06.
Article
em En
| MEDLINE
| ID: mdl-28059103
ABSTRACT
Viral vector mediated gene therapy has become commonplace in clinical trials for a wide range of inherited disorders. Successful gene transfer depends on a number of factors, of which tissue tropism is among the most important. To date, definitive mapping of the spatial and temporal distribution of viral vectors in vivo has generally required postmortem examination of tissue. Here we present two methods for radiolabeling adeno-associated virus (AAV), one of the most commonly used viral vectors for gene therapy trials, and demonstrate their potential usefulness in the development of surrogate markers for vector delivery during the first week after administration. Specifically, we labeled adeno-associated virus serotype 10 expressing the coding sequences for the CLN2 gene implicated in late infantile neuronal ceroid lipofuscinosis with iodine-124. Using direct (Iodogen) and indirect (modified Bolton-Hunter) methods, we observed the vector in the murine brain for up to one week using positron emission tomography. Capsid radioiodination of viral vectors enables non-invasive, whole body, in vivo evaluation of spatial and temporal vector distribution that should inform methods for efficacious gene therapy over a broad range of applications.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Encéfalo
/
Cintilografia
/
Técnicas de Transferência de Genes
/
Dependovirus
/
Proteínas do Capsídeo
/
Vetores Genéticos
/
Radioisótopos do Iodo
Tipo de estudo:
Risk_factors_studies
Limite:
Humans
/
Male
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article