Partial characterization of neural-inducing factors from Xenopus gastrulae Evidence for a larger protein complex containing the factor.
Rouxs Arch Dev Biol
; 201(1): 30-35, 1992 Feb.
Article
em En
| MEDLINE
| ID: mdl-28305609
ABSTRACT
High (Mr ≈ 90-110 kDa) and low (Mr ≈ 15-30 kDa) molecular weight forms of neural-inducing factors have been found in the supernatant of Xenopus gastrula homogenate. The factors, which are protein in nature, have been partially purified by size exclusion high-performance liquid chromatography (HPLC) and sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis. The factor of smaller size, which could be derived from a precursor, is associated with other proteins in a larger complex. The neural-inducing factors are not irreversibly inactivated after chemical deglycosylation with trifluoromethansulfonic acid. The neural-inducing protein which is found in ribonucleoprotein (RNP)-particles was partially purified by hydrophobic chromatography. Possible relationships of the factors in different subcellular fractions and their physiological significance are discussed.
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MEDLINE
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En
Ano de publicação:
1992
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Article