Autoregulated changes in stability of polyribosome-bound beta-tubulin mRNAs are specified by the first 13 translated nucleotides.
Mol Cell Biol
; 8(3): 1224-35, 1988 Mar.
Article
em En
| MEDLINE
| ID: mdl-2835666
ABSTRACT
The expression of tubulin polypeptides in animal cells is controlled by an autoregulatory mechanism whereby increases in the tubulin subunit concentration result in rapid and specific degradation of tubulin mRNAs. We have now determined that the sequences that are necessary and sufficient to specify mouse beta-tubulin mRNAs as substrates for this autoregulated instability reside within the first 13 translated nucleotides (which encode the first four beta-tubulin amino acids Met-Arg-Glu-Ile). This domain has been functionally conserved throughout evolution, inasmuch as sequences isolated from the analogous region of human, chicken, and yeast beta-tubulin mRNAs also confer autoregulation. Further, for an RNA to be a substrate for regulation, not only must it carry the 13-nucleotide coding sequence, but it must also be ribosome bound and its translation must proceed 3' to codon 41.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Polirribossomos
/
Tubulina (Proteína)
/
RNA Mensageiro
/
Regulação da Expressão Gênica
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
1988
Tipo de documento:
Article