Moderate reagent mixing on an orbital shaker reduces the incubation time of enzyme-linked immunosorbent assay.
Anal Biochem
; 528: 53-56, 2017 07 01.
Article
em En
| MEDLINE
| ID: mdl-28416394
ABSTRACT
Rapid diagnostic tests can be developed using ELISA for detection of diseases in emergency conditions. Conventional ELISA takes 1-2 days, making it unsuitable for rapid diagnostics. Here, we report the effect of reagents mixing via shaking or vortexing on the assay timing of ELISA. A 48-min protocol of ELISA involving 12-min incubations with reagent mixing at 750 rpm for every step was optimized. Contrary to this, time-optimized control ELISA performed without mixing produced similar results in 8 h, leaving a time gain of 7 h using the developed protocol. Collectively, the findings suggest the development of ELISA-based rapid diagnostics.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Imunoglobulina G
/
Ensaio de Imunoadsorção Enzimática
Tipo de estudo:
Guideline
Limite:
Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article