Developmental competence of porcine genome-edited zygotes.
Mol Reprod Dev
; 84(9): 814-821, 2017 Sep.
Article
em En
| MEDLINE
| ID: mdl-28471514
ABSTRACT
Genome editing in pigs has tremendous practical applications for biomedicine. The advent of genome editing technology, with its use of site-specific nucleases-including ZFNs, TALENs, and the CRISPR/Cas9 system-has popularized targeted zygote genome editing via one-step microinjection in several mammalian species. Here, we review methods to optimize the developmental competence of genome-edited porcine embryos and strategies to improve the zygote genome-editing efficiency in pigs.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Zigoto
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Animais Geneticamente Modificados
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Sistemas CRISPR-Cas
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Edição de Genes
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article