DRB4 dsRBD1 drives dsRNA recognition in Arabidopsis thaliana tasi/siRNA pathway.
Nucleic Acids Res
; 45(14): 8551-8563, 2017 Aug 21.
Article
em En
| MEDLINE
| ID: mdl-28575480
ABSTRACT
In Arabidopsis thaliana, endogenous trans-acting and exogenous siRNA pathways are initiated by the interaction of DRB4 with trigger dsRNA. Further, DCL4DRB4 complex cleaves the dsRNA into 21 bp siRNA. Understanding molecular determinants and mechanistic details of dsRNA recognition by DRB4 is vital for inducing long-term RNAi-mediated gene regulation in plants. Here, we present solution structures of individual and concatenated DRB4 dsRBDs and demonstrate modes of dsRNA binding by employing NMR, ITC and site-specific mutagenesis. While both dsRBDs adopt the canonical α-ß-ß-ß-α fold, key structural differences and ms-µs dynamics located at the RNA binding region were observed for dsRBD1. These features favor dsRBD1 to orient itself and make stronger tripartite contact with dsRNA, a feature missing in dsRBD2. Additionally, the inter-domain orientation induced by the linker restricts the mobility of dsRBD2, resulting in the steric hindrance of α1 helix in dsRBD2, and leads in further reduction of its dsRNA binding activity. Our study deciphers functional roles of DRB4 domains by showing that dsRBD1 drives the tasiRNA/siRNA pathway. Furthermore, we identify a potential role of the C-terminal region of DRB4 in proteinprotein interaction as it possesses six PxxP motifs, binds to Zn2+ and contains a small structural domain.
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Base de dados:
MEDLINE
Assunto principal:
RNA de Cadeia Dupla
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Proteínas de Ligação a RNA
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Arabidopsis
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RNA de Plantas
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Proteínas de Arabidopsis
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RNA Interferente Pequeno
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article