A jasmonic acid derivative improves skin healing and induces changes in proteoglycan expression and glycosaminoglycan structure.
Biochim Biophys Acta Gen Subj
; 1861(9): 2250-2260, 2017 Sep.
Article
em En
| MEDLINE
| ID: mdl-28602514
ABSTRACT
BACKGROUND:
Jasmonates are plant hormones that exhibit anti-cancer and anti-inflammatory properties and have therefore raised interest for human health applications. The molecular basis of these activities remains poorly understood, although increasing evidence suggests that a variety of mechanisms may be involved. Recently, we have reported that a jasmonate derivative (JAD) displayed anti-aging effects on human skin by inducing extracellular matrix (ECM) remodeling. Based on this observation, we have investigated here the effects of JAD on proteoglycans and glycosaminoglycan (GAG) polysaccharides, which are major cell-surface/ECM components and are involved in a multitude of biological processes. In parallel, we have examined the ability of JAD to promote growth factor activities and improve skin wound healing.METHODS:
Proteoglycan expression was analyzed on epidermal primary keratinocytes and reconstituted skin epidermis, using electron/immunofluorescence microscopy, western blotting and flow cytometry. GAG composition was determined by disaccharide analysis. Finally, biological activities of JAD were assessed in cellulo, in FGF-7 induced migration/proliferation assays, as well as in vivo, using a suction blister model performed on 24 healthy volunteers.RESULTS:
JAD was found to induce expression of major skin proteoglycans and to induce subtle changes in GAG structure. In parallel, we showed that JAD promoted FGF-7 and improved skin healing by accelerating epithelial repair in vivo.CONCLUSION:
This study highlights JAD as a promising compound for investigating GAG structure-function relationships and for applications in skin cosmetic /corrective strategies. GENERALSIGNIFICANCE:
We propose here a novel mechanism, by which jasmonate derivatives may elicit biological activities in mammals.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Reguladores de Crescimento de Plantas
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Proteoglicanas
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Pele
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Cicatrização
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Ciclopentanos
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Oxilipinas
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Glicosaminoglicanos
Tipo de estudo:
Prognostic_studies
Limite:
Adult
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Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article