Direct and tunable modulation of protein levels in rice and wheat with a synthetic small molecule.
Plant Biotechnol J
; 16(2): 472-481, 2018 02.
Article
em En
| MEDLINE
| ID: mdl-28682500
ABSTRACT
Direct control of protein level enables rapid and efficient analyses of gene functions in crops. Previously, we developed the RDDK-Shield1 (Shld1) system in the model plant Arabidopsis thaliana for direct modulation of protein stabilization using a synthetic small molecule. However, it was unclear whether this system is applicable to economically important crops. In this study, we show that the RDDK-Shld1 system enables rapid and tunable control of protein levels in rice and wheat. Accumulation of RDDK fusion proteins can be reversibly and spatio-temporally controlled by the synthetic small-molecule Shld1. Moreover, RDDK-Bar and RDDK-Pid3 fusions confer herbicide and rice blast resistance, respectively, in a Shld1-dependent manner. Therefore, the RDDK-Shld1 system provides a reversible and tunable technique for controlling protein functions and conditional expression of transgenes in crops.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Plantas
/
Oryza
/
Triticum
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article