Induced Pluripotent Stem Cells: Advances in the Quest for Genetic Stability during Reprogramming Process.
Int J Mol Sci
; 18(9)2017 Sep 13.
Article
em En
| MEDLINE
| ID: mdl-28902128
ABSTRACT
Evaluation of the extent and nature of induced pluripotent stem cell (iPSC) genetic instability is important for both basic research and future clinical use. As previously demonstrated regarding embryonic stem cells, such DNA aberrations might affect the differentiation capacity of the cells and increase their tumorigenicity. Here, we first focus on the contribution of multiple DNA damage response pathways during cellular reprogramming. We then discuss the origin and mechanisms responsible for the modification of genetic material in iPSCs (pre-existing variations in somatic cells, mutations induced by reprogramming factors, and mutations induced by culture expansion) and deepen the possible functional consequences of genetic variations in these cells. Lastly, we present some recent improvements of iPSC generation methods aimed at obtaining cells with fewer genetic variations.
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Base de dados:
MEDLINE
Assunto principal:
Instabilidade Genômica
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Reprogramação Celular
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Células-Tronco Pluripotentes Induzidas
Limite:
Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article