Prostaglandin E2 suppresses human group 2 innate lymphoid cell function.
J Allergy Clin Immunol
; 141(5): 1761-1773.e6, 2018 05.
Article
em En
| MEDLINE
| ID: mdl-29217133
ABSTRACT
BACKGROUND:
Group 2 innate lymphoid cells (ILC2s) are involved in the initial phase of type 2 inflammation and can amplify allergic immune responses by orchestrating other type 2 immune cells. Prostaglandin (PG) E2 is a bioactive lipid that plays protective roles in the lung, particularly during allergic inflammation.OBJECTIVE:
We set out to investigate how PGE2 regulates human ILC2 function.METHODS:
The effects of PGE2 on human ILC2 proliferation and intracellular cytokine and transcription factor expression were assessed by means of flow cytometry. Cytokine production was measured by using ELISA, and real-time quantitative PCR was performed to detect PGE2 receptor expression.RESULTS:
PGE2 inhibited GATA-3 expression, as well as production of the type 2 cytokines IL-5 and IL-13, from human tonsillar and blood ILC2s in response to stimulation with a combination of IL-25, IL-33, thymic stromal lymphopoietin, and IL-2. Furthermore, PGE2 downregulated the expression of IL-2 receptor α (CD25). In line with this observation, PGE2 decreased ILC2 proliferation. These effects were mediated by the combined action of E-type prostanoid receptor (EP) 2 and EP4 receptors, which were specifically expressed on ILC2s.CONCLUSION:
Our findings reveal that PGE2 limits ILC2 activation and propose that selective EP2 and EP4 receptor agonists might serve as a promising therapeutic approach in treating allergic diseases by suppressing ILC2 function.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Linfócitos
/
Dinoprostona
/
Regulação da Expressão Gênica
/
Imunidade Inata
Limite:
Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article