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E4BP4 inhibits AngII-induced apoptosis in H9c2 cardiomyoblasts by activating the PI3K-Akt pathway and promoting calcium uptake.
Chen, Bih-Cheng; Shibu, Marthandam Asokan; Kuo, Chia-Hua; Shen, Chia-Yao; Chang-Lee, Shu Nu; Lai, Chao-Hung; Chen, Ray-Jade; Yao, Chun-Hsu; Viswanadha, Vijaya Padma; Liu, Jian-Shen; Chen, Wei-Kung; Huang, Chih-Yang.
Afiliação
  • Chen BC; School of Post-Baccalaureate Chinese Medicine, China Medical University, Taichung, Taiwan.
  • Shibu MA; Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan.
  • Kuo CH; Laboratory of Exercise Biochemistry, University of Taipei, Taipei, Taiwan.
  • Shen CY; Department of Nursing, Meiho University, Pingtung, Taiwan.
  • Chang-Lee SN; Department of Healthcare Administration, Asia University, Taiwan.
  • Lai CH; Division of Cardiology, Department of Internal Medicine, Armed Force Taichung, General Hospital, Taichung 41152, Taiwan.
  • Chen RJ; Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.
  • Yao CH; Department of Biomedical Imaging and Radiological Science, China Medical University, Taichung, Taiwan.
  • Viswanadha VP; Department of Biotechnology, Bharathiar University, Coimbatore 641046, India.
  • Liu JS; Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, China Medical University Beigang Hospital, Yunlin County, Taiwan; Department of Emergency Medicine, China Medical University Hospital, Taichung, Taiwan.
  • Chen WK; School of Post-Baccalaureate Chinese Medicine, China Medical University, Taichung, Taiwan.
  • Huang CY; Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan; Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan; Graduate Institute of Chinese Medical Science, China Medical University, Taichung, Taiwan. Electronic address: cyhuang@mail.cm
Exp Cell Res ; 363(2): 227-234, 2018 02 15.
Article em En | MEDLINE | ID: mdl-29331388
ABSTRACT
The bZIP transcription factor E4BP4 is a survival factor that is known to be elevated in diseased heart and promote cell survival. In this study the role of E4BP4 on angiotensin-II (AngII)-induced apoptosis has been examined in in vitro cell model. H9c2 cardiomyoblast cells that overexpressed E4BP4 were exposed to AngII to observe the cardio-protective effects of E4BP4 on hypertension related apoptosis. The results from TUNEL assays revealed that E4BP4 significantly attenuated AngII-induced apoptosis. Further analysis by Western blot and RT-PCR showed that E4BP4 inhibited AngII-induced IGF-II mRNA expression and cleavage of caspase-3 through the PI3K-Akt pathway. In addition, E4BP4 enhanced calcium reuptake into the sacroplasmic reticulum by down-regulating PP2A and by up-regulating the phosphorylation of PKA and PLB proteins. Our findings indicate that E4BP4 functions as a survival factor in cardiomyoblasts by inhibiting IGF-II transcription and by regulating calcium cycling.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Cálcio / Apoptose / Miócitos Cardíacos / Fatores de Transcrição de Zíper de Leucina Básica Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Cálcio / Apoptose / Miócitos Cardíacos / Fatores de Transcrição de Zíper de Leucina Básica Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article