A Three-Component Organometallic Tyrosine Bioconjugation.
Angew Chem Int Ed Engl
; 57(11): 2827-2830, 2018 03 05.
Article
em En
| MEDLINE
| ID: mdl-29356233
ABSTRACT
Metal-based bioconjugation linkages represent a little-studied approach to protein functionalization that provides novel reactivity, stability, and function. Described is an organometallic bioconjugation, employing rhodium(III) salts, to link boronic acids with tyrosine residues by an arene complex. Both peptides and proteins are amenable to the mild bioconjugation in aqueous media, allowing incorporation of useful functionalities, such as affinity handles or fluorophores. Because of the metastability of the inorganic linkage, the conjugates are susceptible to cleavage by nucleophilic redox mediators but are stable toward typical biological conditions.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Ródio
/
Tirosina
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Ácidos Borônicos
/
Proteínas
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article