Evolved Cas9 variants with broad PAM compatibility and high DNA specificity.

Hu, Johnny H; Miller, Shannon M; Geurts, Maarten H; Tang, Weixin; Chen, Liwei; Sun, Ning; Zeina, Christina M; Gao, Xue; Rees, Holly A; Lin, Zhi; Liu, David R

Hu, Johnny H; Miller, Shannon M; Geurts, Maarten H; Tang, Weixin; Chen, Liwei; Sun, Ning; Zeina, Christina M; Gao, Xue; Rees, Holly A; Lin, Zhi; Liu, David R.

Afiliação

Hu JH; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA.
Miller SM; Howard Hughes Medical Institute, Harvard University, Cambridge, Massachusetts 02138, USA.
Geurts MH; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
Tang W; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA.
Chen L; Howard Hughes Medical Institute, Harvard University, Cambridge, Massachusetts 02138, USA.
Sun N; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
Zeina CM; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA.
Gao X; Howard Hughes Medical Institute, Harvard University, Cambridge, Massachusetts 02138, USA.
Rees HA; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
Lin Z; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA.
Liu DR; Howard Hughes Medical Institute, Harvard University, Cambridge, Massachusetts 02138, USA.

Nature ; 556(7699): 57-63, 2018 04 05.

Article em En | MEDLINE | ID: mdl-29512652

Assuntos

Proteínas Associadas a CRISPR/genética; Proteínas Associadas a CRISPR/metabolismo; Sistemas CRISPR-Cas/genética; DNA/genética; DNA/metabolismo; Edição de Genes/métodos; Mutação; Especificidade por Substrato/genética; Clivagem do DNA; Desoxirribonucleases/metabolismo; Evolução Molecular Direcionada; Genoma Humano/genética; Células HEK293; Humanos; Motivos de Nucleotídeos; Streptococcus pyogenes/enzimologia; Streptococcus pyogenes/genética; Ativação Transcricional

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Especificidade por Substrato / DNA / Proteínas Associadas a CRISPR / Sistemas CRISPR-Cas / Edição de Genes / Mutação Limite: Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article

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