TANGO1 builds a machine for collagen export by recruiting and spatially organizing COPII, tethers and membranes.
Elife
; 72018 03 07.
Article
em En
| MEDLINE
| ID: mdl-29513218
ABSTRACT
Collagen export from the endoplasmic reticulum (ER) requires TANGO1, COPII coats, and retrograde fusion of ERGIC membranes. How do these components come together to produce a transport carrier commensurate with the bulky cargo collagen? TANGO1 is known to form a ring that corrals COPII coats, and we show here how this ring or fence is assembled. Our data reveal that a TANGO1 ring is organized by its radial interaction with COPII, and lateral interactions with cTAGE5, TANGO1-short or itself. Of particular interest is the finding that TANGO1 recruits ERGIC membranes for collagen export via the NRZ (NBAS/RINT1/ZW10) tether complex. Therefore, TANGO1 couples retrograde membrane flow to anterograde cargo transport. Without the NRZ complex, the TANGO1 ring does not assemble, suggesting its role in nucleating or stabilising this process. Thus, coordinated capture of COPII coats, cTAGE5, TANGO1-short, and tethers by TANGO1 assembles a collagen export machine at the ER.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Transporte Proteico
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Retículo Endoplasmático
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Translocador Nuclear Receptor Aril Hidrocarboneto
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Antígenos de Neoplasias
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Proteínas de Neoplasias
Limite:
Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article